Application of Clostridium butyricum in ruminants

Application of Clostridium butyricum in ruminants丁酸梭菌在反刍动物中的应用丁酸梭菌在反刍动物中的应用

1. Adjusting the balance of gut microbiota1. 调节肠道菌群的平衡1. 调节肠道菌群的平衡

      Clostridium butyricum 丁酸梭菌 has a dual function of preventing the abnormal proliferation and development of pathogenic and spoilage bacteria in the intestine, thereby correcting the imbalance of intestinal flora and reducing the production of enterotoxins. Clostridium butyricum has a biological antagonistic effect on Clostridium welchii, and it has been preliminarily confirmed that it inhibits the expression of toxic proteins through tight contact with cells. The inhibitory mechanism still needs further research. When two types of bacteria are co cultured in vitro, the toxicity of Clostridium difficile is significantly reduced or even disappeared. As long as the germination and expansion rate of Clostridium butyricum spores is twice that of Clostridium difficile, the short chain fatty acids produced are more effective in inhibiting the germination and growth of Clostridium difficile spores. Research by Chen Qiuhong and others has shown that Clostridium butyricum has strong biological characteristics of high temperature resistance, acid resistance, and bile salt resistance. It has a strong inhibitory effect on intestinal pathogenic bacteria such as Escherichia coli, Salmonella, and Shigella, while significantly promoting the growth of beneficial bacteria in the intestine. The fermented extract of Clostridium butyricum increased the live bacterial count of Lactobacillus acidophilus, Bifidobacterium, and Streptococcus faecalis compared to the control group.

丁酸梭菌具有防止肠道内致病菌和腐败菌异常增殖发育的双重功能，从而纠正肠道菌群失衡，减少肠毒素的产生。丁酸梭菌对韦氏梭菌具有生物拮抗作用，初步证实其通过与细胞紧密接触抑制毒性蛋白的表达。其抑制机制仍需进一步研究。当两种细菌在体外共培养时，艰难梭菌的毒性明显降低甚至消失。只要丁酸梭菌孢子的萌发和膨胀率是艰难梭菌的两倍，产生的短链脂肪酸对艰难梭菌孢子的萌发和生长有更有效的抑制作用。陈秋红等人的研究表明，丁酸梭菌具有较强的耐高温、耐酸、耐胆盐等生物学特性。对大肠杆菌、沙门氏菌、志贺氏菌等肠道致病菌有较强的抑制作用，同时显著促进肠道有益菌的生长。与对照组相比，丁酸梭菌发酵提取物增加了嗜酸乳杆菌、双歧杆菌和粪链球菌的活菌数量。

2. Enhance immune function and promote animal growth2. 增强免疫功能，促进动物生长

      In clinical applications, vaccines made from heat inactivated Clostridium butyricum have the effect of activating macrophages and NK cells. Oral administration of Clostridium butyricum significantly increases serum IgA and IgM levels. Fermentation of animal feed with Clostridium butyricum can reduce anti nutritional factors in feed, increase nutrients such as amino acids, vitamins, organic acids, and organic phosphorus, and improve palatability, intake, and utilization efficiency; Adding to animal feed has the potential to replace antibiotics in the prevention and treatment of related animal diseases, inhibit the occurrence of endogenous diseases, enhance the body's immunity and the number of white blood cells in the blood, improve animal production performance and product quality, reduce the use of antibiotics, and increase economic efficiency. Adding 1% Clostridium butyricum to the diet of weaned piglets significantly increased their average daily weight gain. Adding 3% Clostridium butyricum significantly improved the conversion efficiency of piglet feed. Adding 3% and 5% Clostridium butyricum significantly improved their immune function and disease resistance. Among them, the activity of serum lactate dehydrogenase in the group adding 5% Clostridium butyricum was significantly lower than the IgG level in the antibiotic control group, and the C3 level was significantly higher than that in the antibiotic control group. Adding a certain amount of Clostridium butyricum to the feed of Miiu fish significantly increased the activity of serum phenolic oxidase and acid phosphatase, as well as the activity of lysozyme and IgM levels in serum and body surface mucus (P<0.05). As well as its significant increase in growth, Clostridium butyricum can regulate the humoral immune response and improve its growth performance in the fish.在临床应用中，由热灭活丁酸梭菌制成的疫苗具有活化巨噬细胞和NK细胞的作用。口服丁酸梭菌可显著提高血清IgA和IgM水平。用丁酸梭菌发酵动物饲料，可降低饲料中的抗营养因子，增加饲料中氨基酸、维生素、有机酸、有机磷等营养物质，提高适口性、采食量和利用效率;在动物饲料中添加抗生素，在预防和治疗相关动物疾病、抑制内源性疾病的发生、增强机体免疫力和血液中白细胞数量、提高动物生产性能和产品质量、减少抗生素使用、提高经济效益等方面具有替代抗生素的潜力。饲粮中添加1%丁酸梭菌显著提高断奶仔猪平均日增重。添加3%丁酸梭菌可显著提高仔猪饲料的转化效率。添加3%和5%丁酸梭菌可显著提高鸡的免疫功能和抗病性。其中，添加5%丁酸梭菌组血清乳酸脱氢酶活性显著低于抗生素对照组IgG水平，C3水平显著高于抗生素对照组。饲料中添加一定量的丁酸梭菌显著提高了蛋鱼血清酚氧化酶和酸性磷酸酶活性，以及血清和体表黏液中溶菌酶活性和IgM水平(P<0.05)。丁酸梭菌在显著促进鱼生长的同时，还能调节体液免疫反应，提高鱼的生长性能。

3. Produce probiotics 3. 生产益生菌

      The main metabolite of Clostridium butyricum is butyric acid, which is the main nutrient for energy metabolism and normal growth of colonic epithelial cells. Insufficient energy supply is one of the main factors leading to colitis, and 70% of energy in colonic epithelial cells is generally obtained from butyrate. Glucose sulfate can inhibit butyrate oxidation without affecting glucose metabolism. Oral administration of glucose sulfate in mice can cause colitis.丁酸梭菌的主要代谢产物是丁酸，是能量代谢和结肠上皮细胞正常生长的主要营养物质。能量供应不足是导致结肠炎的主要因素之一，结肠上皮细胞70%的能量一般来自丁酸盐。硫酸葡萄糖可以抑制丁酸盐氧化，但不影响葡萄糖代谢。小鼠口服硫酸葡萄糖可引起结肠炎。

Product efficacy: 产品功效:

      1. Promote the proliferation and development of beneficial gut microbiota (Bifidobacterium, Lactobacillus) in animals, inhibit the growth and reproduction of harmful and spoilage bacteria in the intestine, correct gut microbiota disorders, and reduce the occurrence of enterotoxins; It can act as a multi bacterium;1. 促进动物有益肠道菌群(双歧杆菌、乳酸菌)的增殖发育，抑制肠道内有害和腐败菌的生长繁殖，纠正肠道菌群紊乱，减少肠毒素的发生;它可以作为一个多细菌;

      2. It can produce B vitamins, vitamin K, amylase and other substances in the animal intestines, which have health benefits;2. 能在动物肠道内产生B族维生素、维生素K、淀粉酶等物质，对健康有益;

      3. Clostridium butyricum 丁酸梭菌 is a probiotic that can repair intestinal mucosa. Its main metabolite, butyric acid, is the main nutrient for the regeneration and repair of intestinal epithelial tissue cells;3. 丁酸梭菌是一种具有修复肠道黏膜功能的益生菌。其主要代谢物丁酸是肠上皮组织细胞再生修复的主要营养物质;

      4. Anaerobic or facultative anaerobic Bacillus, unaffected by gastric acid, bile acid, etc;4. 厌氧或兼性厌氧杆菌，不受胃酸、胆汁酸等影响;

      5. Has strong tolerance to various feed antibiotics and can be used in combination. The use of Clostridium butyricum as a new feed additive is of great significance in reducing the abuse of antibiotic products in current feed, reducing drug residues in meat, reducing the resistance of animal bacteria, and ensuring animal health.5. 对各种饲料抗生素有较强的耐受性，可组合使用。使用丁酸梭菌作为新型饲料添加剂，对于减少当前饲料中抗生素产品的滥用，减少肉类中的药物残留，降低动物细菌的耐药性，保证动物健康具有重要意义。

Recommended dosage: 建议用量:

Poultry: 200g of this product added per ton of feed家禽:每吨饲料中添加200克本品

Adult poultry: Add 50-100g of this product per ton of feed成年家禽:每吨饲料添加50-100g本品

Piglets: 200g of this product added per ton of feed仔猪:每吨饲料添加200克本品

Sows: 200g of this product added per ton of feed母猪:每吨饲料添加200克本品

Aquatic products: Add 100-200g of this product per ton of feed水产品:每吨饲料中添加100-200g本品

Fattening pigs: Add 50-150g of this product per ton of feed育肥猪:每吨饲料中添加50-150g本品

Ruminant animals: Add 100-200g of this product per ton of feed反刍动物:每吨饲料添加100-200g本品

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