Specific usage methods of swamp red pseudomonas in aquaculture

      The swamp red pseudomonas can improve the aquaculture effect by improving the water environment, providing nutrients, and enhancing the immune system of aquaculture subjects. The specific usage method needs to be adjusted according to the aquaculture scenario, aquaculture subjects, and water quality. The following are common application methods:

1、 Water splashing method (core usage method)

      By directly spraying bacterial liquid or agents onto aquaculture water bodies, water quality can be quickly regulated and harmful microorganisms can be inhibited, making it suitable for scenarios such as ponds, net cages, and industrial aquaculture.

Timing of use:

      In the early stage of breeding, sprinkle 3-5 days before releasing the seedlings, purify the water in advance, establish a beneficial bacterial environment, and reduce the stress response of the seedlings.

      Mid stage aquaculture: Sprinkle once every 7-15 days, especially during high temperature periods and rainy weather (water bodies are prone to deterioration), to prevent the increase of ammonia nitrogen and nitrite, and reduce the outbreak of harmful algae such as blue-green algae and dinoflagellates.

      When water quality deteriorates: When there is an odor, turbidity, or abnormal pH (such as pH>8.5 or<6.5) in the water body, or when ammonia nitrogen ≥ 0.5mg/L and nitrite ≥ 0.1mg/L are detected, the dosage should be doubled and sprayed to quickly improve water quality.

Dosage used:

      Routine maintenance: Use 500-1000mL of bacterial solution with a concentration of 10 ^ 8-10 ^ 9 CFU/mL or 100-200g of solid bacterial agent (with a bacterial content ≥ 10 ^ 10 CFU/g) per acre (water depth of 1 meter), dilute 100-200 times, and evenly sprinkle the entire pool.

      Water quality emergency: The dosage is doubled, and the effect is even better if combined with zeolite powder (adsorbing harmful substances) or Bacillus subtilis (synergistically decomposing organic matter).

matters needing attention:

      Choose to sprinkle on sunny days from 9-11am, when the light is suitable for the growth of photosynthetic bacteria; Avoid using on rainy days or in the evening (due to lack of oxygen).

      Turn on the aerator for 1-2 hours before sprinkling to increase dissolved oxygen in the water; Maintain a dissolved oxygen level of ≥ 5mg/L within 24 hours after sprinkling to prevent hypoxia.

      Interval use with chemical disinfectants and antibiotics: If disinfectants have been used before, they should be sprayed 3-5 days later (until the drug disappears); If you sprinkle the microbial agent first, you need to wait 2-3 days before using the chemical agent to avoid killing beneficial bacteria.

2、 Feed mixing method (supplementary nutrition)

      Mix swamp red pseudomonas with feed and use its high protein (containing 60% -70% protein), amino acids, vitamins, and bioactive substances (such as carotenoids and coenzyme Q10) to enhance the growth rate and immunity of aquaculture subjects.

usage method:

      Bacterial liquid mixing: Mix the bacterial liquid with a concentration of 10 ^ 8 CFU/mL and feed in a ratio of 1:10-1:20 (such as adding 5-10mL of bacterial liquid to 100g of feed), mix well, and dry in the shade (to avoid direct sunlight damaging the bacterial cells). Mix and feed on site.

      Bacterial powder mixing: Solid bacterial powder (with a bacterial content of ≥ 10 ^ 10 CFU/g) is added at a rate of 0.1% -0.5% of the feed weight, thoroughly mixed with the feed, and fed.

Applicable to:

      Seedling stage: Shrimp and fish fry have high nutritional requirements, and mixed feeding can improve survival rates (such as South American white shrimp and tilapia fry).

      Adult aquaculture: Regular feeding of fish (such as grass carp and sea bass) and crustaceans (such as river crabs and crayfish) can reduce diseases (such as gill rot and enteritis) and promote growth.

      Usage frequency: Feed once every 3-5 days, or stop feeding for 2-3 days after continuous feeding for 3-5 days, to avoid long-term single use leading to nutritional imbalance.

3、 Seed soaking method (to enhance seedling vitality)

      Soak the seedlings in water containing swamp red pseudomonas before planting, and improve their stress resistance by colonizing beneficial bacteria and reducing pathogen attachment on the surface.

Operation steps:

      Preparation of bacterial solution: Dilute the swamp red pseudomonas bacterial solution to a concentration of 10 ^ 7-10 ^ 8 CFU/mL (a small amount of salt can be added to adjust osmotic pressure).

      Soaking time: Adjust according to the size of the seedlings. Shrimp and fish fry are generally soaked for 15-30 minutes, while crab and shellfish fry can be extended to 1-2 hours (during which they are inflated and oxygenated to avoid hypoxia).

      Advantages: Reduce the stress response of seedlings after transportation, lower the probability of disease after entering the pond, especially suitable for high-density seedling cultivation scenarios.

4、 Use in conjunction with other measures to enhance effectiveness

      Paired with organic/inorganic fertilizers: Apply a small amount of organic fertilizer (such as chicken manure and soybean cake) 1-2 days before spraying the microbial agent to provide carbon sources and nutrients for photosynthetic bacteria, promoting their reproduction; If the fertility of the water body is insufficient, a small amount of nitrogen fertilizer (such as urea) and phosphorus fertilizer (such as potassium dihydrogen phosphate) can be combined to improve the activity of the microbial agent.

      Synergistic with other probiotics: When used in combination with Bacillus, Lactobacillus, EM bacteria, etc. (with an interval of 1-2 days), a complementary bacterial community is formed - Pseudomonas aeruginosa is good at degrading small molecule organic matter and improving bottom dissolved oxygen, Bacillus decomposes large molecule organic matter, and Lactobacillus inhibits harmful intestinal bacteria, improving overall water quality and the health of aquaculture subjects.

      Bottom improvement assistance: For ponds with deteriorating bottom quality (blackening, odorous), bottom improvement agents (such as potassium hydrogen persulfate) can be used to oxidize harmful substances in the bottom layer. After 3 days, swamp red pseudomonas can be sprinkled to accelerate the decomposition of organic matter in the sediment and reduce the release of ammonia nitrogen and hydrogen sulfide.

5、 Precautions

      Storage conditions: The microbial agent should be stored away from light and at low temperatures (2-8 ℃), avoiding direct sunlight and high temperatures. Before use, it should be taken out in advance and restored to room temperature to prevent temperature difference stimulation.

      Dosage control: Overuse may cause a slight increase in water pH (alkaline substances produced by photosynthetic bacteria metabolism), and regular water quality testing is necessary. If pH>9.0, use should be suspended and water should be changed for adjustment.

      Taboo scenario: Do not use when the water is severely lacking in oxygen (dissolved oxygen<3mg/L), oxygen should be added first; When acute diseases occur in breeding objects, targeted treatment should be given first, and bacterial agents should be used for adjustment after the condition stabilizes.

      Through the above methods, swamp red pseudomonas can effectively reduce the incidence of "algal blooms" and diseases in aquaculture, while reducing the use of antibiotics, making it suitable for green ecological aquaculture models. In practical applications, it is necessary to flexibly adjust according to the scale of aquaculture and water quality monitoring data to achieve the best results.